Unlock Precision Genetics with Geneprint’s Amplicon Sequencing Solutions

At Geneprint, we bring cutting-edge Amplicon Sequencing (Amplicon-Seq) technology to research labs, healthcare providers, and biotech companies. Our targeted sequencing platform offers a fast, accurate, and cost-effective method for analyzing genetic variation within specific genomic regions.

What is Amplicon Sequencing?

Amplicon Sequencing is a targeted NGS (Next-Generation Sequencing) method that uses PCR to amplify specific DNA regions of interest before sequencing. It is ideal for analyzing mutations, SNPs, or indels in genes relevant to cancer, inherited disorders, microbial communities, or other areas of research.

Applications of Amplicon Sequencing

• Cancer Genomics
  Detect mutations in oncogenes or tumor suppressor genes (e.g., BRCA1/2, TP53).

• Microbiome Profiling
  Sequence 16S rRNA or ITS regions for microbial identification and diversity analysis.

• Inherited Disease Screening
  Identify disease-related variants in targeted genes.

• Gene Editing Validation
  Assess CRISPR/Cas9 or other gene editing outcomes.

• Pathogen Detection
  Identify viral or bacterial genomes in clinical or environmental samples.

Why Choose Geneprint for Amplicon Sequencing?

• Custom Panel Design
  We help you design gene panels tailored to your research goals.

• High Throughput, High Accuracy
  Fast turnaround with deep coverage and accurate variant detection.

• End-to-End Support
  From sample QC and library prep to bioinformatics analysis and interpretation.

• Compatible with Low DNA Input
  Suitable for FFPE samples, liquid biopsies, and degraded DNA.

• Secure & Confidential
  We follow strict data protection protocols for all client data.

Workflow at Geneprint

1. Panel Selection / Customization
   Choose from ready-to-use panels or design your own.

2. Sample Submission & Quality Control
   We accept gDNA, FFPE, cfDNA, or microbial DNA samples.

3. Library Preparation & Sequencing
   Amplification using PCR followed by Illumina/ION sequencing.

4. Bioinformatics & Reporting
   Variant calling, annotation, and data interpretation included.

Sample Requirements

• DNA Input: 10–50 ng (gDNA, FFPE, cfDNA accepted)

• Quality: A260/280 ratio of 1.8–2.0

• Format: Tubes or 96-well plates

• Storage: Store samples at -20°C

Turnaround Time

5–10 business days
(Depending on panel size and data complexity)

Frequently Asked Questions

Q1. Can I customize my own amplicon panel?
Yes! We offer full support in designing custom gene panels for your specific research needs.

Q2. What sequencing platforms do you use?
We primarily use Illumina MiSeq and Ion Torrent systems for high-accuracy results.

Q3. Do you provide bioinformatics support?
Absolutely. Our reports include raw data, processed data (FASTQ/VCF), variant interpretation, and visualizations.

Q4. What file formats will I receive?
We provide FASTQ, VCF, and detailed PDF summary reports. Custom output formats are also available on request.